H-start for exclusively heteronuclear NMR spectroscopy: The case of intrinsically disordered proteins

  • Bermel W
  • Bertini I
  • Csizmok V
 et al. 
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Abstract

Here, we present a series of exclusively heteronuclear multidimensional NMR experiments, based on13C direct detection, which exploit the1H polarization as a starting source to increase the signal-to-noise ratio. This contributes to make this spectroscopy more useful and usable. Examples are reported for a suitable system such as securin, an intrinsically disordered protein of 22 kDa. © 2009 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • 13C direct detection
  • IPAP
  • Intrinsically disordered proteins
  • Protonless NMR
  • Spin-state selection
  • Virtual decoupling

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