Here, we present a series of exclusively heteronuclear multidimensional NMR experiments, based on13C direct detection, which exploit the1H polarization as a starting source to increase the signal-to-noise ratio. This contributes to make this spectroscopy more useful and usable. Examples are reported for a suitable system such as securin, an intrinsically disordered protein of 22 kDa. © 2009 Elsevier Inc. All rights reserved.
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