Hydroxy and methoxy substituted thiacarbocyanines for fluorescent detection of amyloid formations

  • Volkova K
  • Kovalska V
  • Losytskyy M
 et al. 
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Abstract

In present paper series of trimethine cyanines modified in 5,5'- or 6,6'- position with hydroxy- or methoxy- substituents is studied for their ability to interact selectively with fibrillar formations. Processes of dye aggregation that accompany this interaction were also investigated. Meso-methyl trimethynecyanines with 5,5'- methoxy (7519) and hydroxy (7515) substituents strongly (up to 40 times) increase fluorescence intensity in the presence of fibrillar insulin, and also give noticeable fluorescent response on the presence of various aggregated proteins (lysozyme, beta-lactoglobulin, alpha-synuclein A53T). 7519 and 7515 dyes can be used for fluorometric detection of fibrillar insulin at concentrations of approximately 1.5-120 microg/ml. For meso-ethyl substituted dye 7514 the ability to form H- and J-aggregates upon interaction with insulin fibrils was suggested. The model of the H- and J-aggregate packing in the protein fibrillar structure has been proposed.

Author-supplied keywords

  • Amyloid proteins
  • Cyanine dyes
  • Fluorescent detection
  • J-aggregates

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Authors

  • Kateryna D. Volkova

  • Vladyslava B. Kovalska

  • Mykhaylo Yu Losytskyy

  • Kateryna O. Fal

  • Nadiya O. Derevyanko

  • Yuriy L. Slominskii

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