Hypoxic and pharmacological preconditioning preserves vasomotor response of porcine coronary artery.

  • Kuzner J
  • Drevensek G
  • Gersak B
 et al. 
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Abstract

Vasomotor response of the coronary artery depends on both endothelial and smooth muscle cells. Response is altered by hypoxia-reoxygenation-induced damages. Hypoxic preconditioning and pharmacological preconditioning as well can prevent these alterations. We compared the effectiveness of both types of preconditioning against hypoxia-reoxygenation-induced changes in vasomotor response of the isolated artery. Porcine arterial rings (3-4 mm wide) were cut from the left anterior descending porcine coronary artery and placed in Krebs-Henseleit solution. In order to obtain control response of the arteries, we contracted arterial rings with 20 mM KCl before ("standard contraction") and after 60-min hypoxia and 30-min reoxygenation. In other groups, nitric oxide-synthase and cyclooxygenase were inhibited. Then, the rings were pre-contracted with U46619 and relaxed by cumulative addition of the substance P. Contractions and relaxations of non-preconditioned and hypoxically or pharmacologically preconditioned rings were compared. Hypoxic preconditioning was performed by two periods of 5-min hypoxia and 10-min reoxygenation. For pharmacological preconditioning, we used application of adenosine, adrenaline, acetylcholine and angiotensin II. Analysis was performed with one-way ANOVA, followed by Dunnett's Multiple Comparison Test. After hypoxia-reoxygenation, in non-preconditioned rings KCl-induced contractions were significantly increased compared to standard contraction. Relaxations of hypoxically and pharmacologically preconditioned rings (expressed as percentages of U46619-induced pre-contraction) were significantly decreased (p < 0.01) compared to hypoxic but not to normoxic rings. Hypoxic and pharmacological preconditioning may preserve contraction and endothelium-dependent relaxation of porcine coronary artery after long-lasting hypoxia-reoxygenation.

Author-supplied keywords

  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5
  • Acetylcholine
  • Acetylcholine: pharmacology
  • Adenosine
  • Adenosine: pharmacology
  • Angiotensin II
  • Angiotensin II: pharmacology
  • Animals
  • Coronary Vessels
  • Coronary Vessels: drug effects
  • Coronary Vessels: physiology
  • Epinephrine
  • Epinephrine: pharmacology
  • Nitroarginine
  • Nitroarginine: pharmacology
  • Oxygen
  • Oxygen: pharmacology
  • Substance P
  • Substance P: pharmacology
  • Swine
  • Vasoconstriction
  • Vasoconstriction: drug effects
  • Vasodilation
  • Vasodilation: drug effects

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  • PUI: 40287481
  • ISSN: 1230-6002
  • SCOPUS: 2-s2.0-14244263020
  • SGR: 14244263020
  • PMID: 15662092
  • ISBN: 1230-6002

Authors

  • Jernej Kuzner

  • Gorazd Drevensek

  • Borut Gersak

  • Metka Budihna

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