Identification of bioactive constituents of Ziziphus jujube fruit extracts exerting antiproliferative and apoptotic effects in human breast cancer cells

  • Plastina P
  • Bonofiglio D
  • Vizza D
 et al. 
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Abstract

Ziziphus exts. have been used in Traditional Chinese Medicine for the treatment of cancer. In the present study we have investigated the effects of Ziziphus jujube exts. (ZEs) on breast cancer. We evaluated the effects of increasing concns. of ZEs on ER�� pos. MCF-7 and ER�� neg. SKBR3 breast cancer cell proliferation using MTT assays. Apoptosis was analyzed by evaluating the involvement of some pro-apoptotic proteins, including Bax, Bad, Bid and PARP cleavage by immunoblotting anal. Moreover, the effects of ZEs treatment on apoptosis were tested by both DNA fragmentation and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining. By using chromatog. techniques, we identified the constituents of the effective exts. ZE1, ZE2, and ZE4 exerted significant antiproliferative effects on estrogen receptor alpha (ER��) pos. MCF-7 (IC50 values of 14.42, 7.64, 1.69 ׬g/mL) and ER�� neg. SKBR3 (IC50 values of 14.06, 6.21, 3.70 ׬g/mL) human breast cancer cells. Remarkably, ZEs did not affect cell viability of both normal human fibroblasts BJ1-hTERT and nonmalignant breast epithelial MCF-10A cells. Treatment with ZEs induced cell death by apoptosis in both malignant breast cells. We found that the most effective exts. ZE2 and ZE4 shared a no. of triterpenic acids, already known for their anticancer activities. Our data provide a rational base for the use of Ziziphus exts. in the treatment of breast cancer in Traditional Chinese Medicine. [on SciFinder(R)]

Author-supplied keywords

  • antitumor apoptosis Ziziphus fruit breast cancer

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Authors

  • Pierluigi Plastina

  • Daniela Bonofiglio

  • Donatella Vizza

  • Alessia Fazio

  • Daniela Rovito

  • Cinzia Giordano

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