Identification of broad specificity P450CAM variants by primary screening against indole as substrate

  • Çelik A
  • Speight R
  • Turner N
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Abstract

High-throughput screening of cytochrome P450CAM libraries, for their ability to oxidise indole to indigo and indirubin, has resulted in the identification of variants with activity towards the structurally unrelated substrate diphenylmethane.

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