Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E

  • Schiavo G
  • Rossetto O
  • Catsicas S
 et al. 
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Abstract

Botulinum neurotoxins are metalloproteins with one zinc atom bound to the zinc binding motif of zinc endopeptidases. Here we show that botulinum neurotoxin serotypes A, D, and E are zinc endoproteases specific for components of the synaptic vesicle docking and fusion complex. Serotypes A and E cleave SNAP-25, a 25-kDa protein of the synaptic terminal, while serotype D is specific for VAMP/synaptobrevin, a membrane protein of synaptic vesicles. Both rat brain VAMP isoforms are cleaved at a single Lys-Leu peptide bond. The proteolytic activity of these neurotoxins is inhibited by EDTA and captopril.

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  • ISBN: 0021-9258
  • ISSN: 00219258
  • SCOPUS: 2-s2.0-0027366557
  • PUI: 23988585
  • PMID: 8226912
  • SGR: 0027366557

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