Identification and in silico analysis of the Citrus HSP70 molecular chaperone gene family

  • Fietto L
  • Costa M
  • Cruz C
 et al. 
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Abstract

The completion of the genome sequencing of the Arabidopsis thaliana
model system provided a powerful molecular tool for comparative analysis
of gene families present in the genome of economically relevant plant
species. In this investigation, we used the sequences of the Arabidopsis
Hsp70 gene family to identify and annotate the Citrus Hsp70 genes
represented in the CitEST database. Based on sequence comparison
analysis, we identified 18 clusters that were further divided into 5
subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s,
one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight
cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital
Northern of each Hsp70 transcript in different organs and in response to
stress conditions. The EST database revealed a distinct population
distribution of Hsp70 ESTs among isoforms and across the organs
surveyed. The Hsp70-5 isoform was highly expressed in seeds, whereas
BiP, mitochondrial and plastid HSp70 mRNAs displayed a similar
expression profile in the organs analyzed, and were predominantly
represented in flowers. Distinct Hsp70 mRNAs were also differentially
expressed during Xylella infection and Citrus tristeza viral infection
as well as during water deficit. This in silico study sets the
groundwork for future investigations to fully characterize functionally
the Citrus Hsp70 family and underscores the relevance of Hsp70s in
response to abiotic and biotic stresses in Citrus.

Author-supplied keywords

  • Chaperones
  • Digital northern
  • Expressed sequence tags
  • Genome
  • Heat shock proteins

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Authors

  • Lucinao G. Fietto

  • Maximiller D L Costa

  • Cosme D. Cruz

  • Alessandra A. Souza

  • Marcos Antonio Machado

  • Elizabeth P B Fontes

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