The objectives of this study were to isolate and physically localize expressed resistance (R) genes on wheat chromosomes. Irrespective of the host or pest type, most of the 46 cloned R genes from 12 plant species share a strong sequence similarity, especially for protein domains and motifs. By utilizing this structural similarity to perform modified RNA fingerprinting and data mining, we identified 184 putative expressed R genes of wheat. These include 87 NB/LRR types, 16 receptor-like kinases, and 13 Pto-like kinases. The remaining were seven Hm1 and two Hs1pro-1 homologs, 17 pathogenicity related, and 42 unique NB/kinases. About 76% of the expressed R-gene candidates were rare transcripts, including 42 novel sequences. Physical mapping of 121 candidate R-gene sequences using 339 deletion lines localized 310 loci to 26 chromosomal regions encompassing ∼16% of the wheat genome. Five major R-gene clusters that spanned only ∼3% of the wheat genome but contained ∼47% of the candidate R genes were observed. Comparative mapping localized 91% (82 of 90) of the phenotypically characterized R genes to 18 regions where 118 of the R-gene sequences mapped.
CITATION STYLE
Dilbirligi, M., Erayman, M., Sandhu, D., Sidhu, D., & Gill, K. S. (2004). Identification of Wheat Chromosomal Regions Containing Expressed Resistance Genes. Genetics, 166(1), 461–481. https://doi.org/10.1534/genetics.166.1.461
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