We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method—termed photoactivated localization microscopy—to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
CITATION STYLE
Lippincott-Schwartz, J., Davidson, M. W., Bonifacino, J. S., Patterson, G. H., Betzig, E., Olenych, S., … Lindwasser, O. W. (2006). Imaging Intracellular Fluorescent Proteins at Nanometer Resolution. Science. Retrieved from http://science.sciencemag.org/content/313/5793/1642.abstract%0Apapers3://publication/doi/10.1126/science.1127344
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