Immune cell inflammatory cytokine responses differ between central and systemic compartments in response to acute exercise in mice

  • Pervaiz N
  • Hoffman-Goetz L
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Background: Exhaustive exercise induces apoptosis and oxidative stress in systemic organs and tissues and is associated with increased levels of pro-inflammatory cytokines. The effects of acute exercise on cytokine expression and apoptosis of immune cells in the central nervous system (CNS) have not been well characterized. Purpose: We investigated the effects of a single bout of strenuous exercise on the expression of TNF-α, IL-6, and IL-β, as well as the apoptotic status of cells in the hippocampus of healthy mice. To compare central vs. systemic differences, cytokine expression in the intestinal lymphocytes of a subset of mice were also assessed. Methods: Female C57BL/6 mice were divided into three groups: sedentary controls (NOTREAD) (n = 22), treadmill exercise with immediate sacrifice (TREADImm) (n = 21), or treadmill exercise with sacrifice after 2 hours (TREAD-2h). TNF-α, IL-6, and IL-1β expression in the hippocampus and intestinal lymphocytes were measured by Western blot analysis. Percentages of hippocampal cells undergoing apoptosis (Annexin+) or necrosis (Propidium Iodide+) were determined through flow cytometry. Plasma levels of 8-isoprostane and corticosterone were measured using commercially available EIA kits. Results: Acute treadmill exercise led to significant decreases in TNF-α (p

Author-supplied keywords

  • Acute exercise
  • Apoptosis
  • Cytokines
  • Hippocampus
  • Intestinal lymphocytes
  • Oxidative stress

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  • Nabeel Pervaiz

  • Laurie Hoffman-Goetz

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