Impact of UV radiation on the production patterns and composition of dissolved free and combined amino acids in marine phytoplankton

  • Goes J
  • Handa N
  • Taguchi S
 et al. 
  • 21

    Readers

    Mendeley users who have this article in their library.
  • 34

    Citations

    Citations of this article.

Abstract

Using the C-13 tracer technique in conjunction with gas chromatographic-mass
spectrometric (GC-MS) techniques, we examined the patterns of synthesis
and the composition of dissolved free and combined amino acids within
phytoplankton photosynthesizing in the presence and absence of natural
solar ultraviolet radiation (UVR). At levels that still permitted
the uptake of carbon assimilation into the cells, WR caused a marked
decline in the overall rate of cart,on incorporated into amino acids
and a reduction in the pool size of total cellular amino acids (TCAA).
In contrast, absolute concentrations of amino acids within the intracellular
dissolved free amino acid (INDFAA) pool (measured using an amino
acid analyzer) were higher in the presence of UVR. An examination
of the production patterns and composition of amino acids constituting
the INDFAA and TCAA pools revealed a marked diminution in the synthesis
and accumulation of alanine and valine in the presence of UVR. On
the other hand, the rates of synthesis and concentrations of glutamic
acid (glutamic acid + glutamine) in the INDFAA and TCAA pools of
phytoplankton were higher in samples exposed to UVR. These changes
are discussed with reference to the known effects of UVR on nitrogen
and carbon assimilation within phytoplankton

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free