The impact of vehicle on the relative potency of skin-sensitizing chemicals in the local lymph node assay

  • Jowsey I
  • Clapp C
  • Safford B
 et al. 
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The identification and characterization of chemicals that possess skin-sensitizing potential are typically performed using predictive tests. However, human exposure to skin-sensitizing chemicals often occurs via a matrix (vehicle) that differs from that used in these tests. It is thus important to account for the potential impact of vehicle differences when undertaking quantitative risk assessment for skin sensitization. This is achieved through the application of a specific sensitization assessment factor (SAF), scaled between 1 and 10, when identifying an acceptable exposure level. The objective of the analysis described herein is to determine the impact of vehicle differences on local lymph node assay (LLNA) EC3 values (concentrations of test chemical required to provoke a 3-fold increase in lymph node cell proliferation). Initially, the inherent variability of the LLNA was investigated by examining the reproducibility of EC3 values for 14 chemicals that have been tested more than once in the same vehicle (4:1 acetone:olive oil, AOO). This analysis reveals that the variability in EC3 value for these chemicals following multiple assessments is

Author-supplied keywords

  • LLNA
  • Sensitization assessment factor
  • Skin-sensitizing potency
  • Vehicle effects

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  • Ian R. Jowsey

  • Catherine J. Clapp

  • Bob Safford

  • Ben T. Gibbons

  • David A. Basketter

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