An improved surface passivation method for single-molecule studies

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Abstract

We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.

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Hua, B., Han, K. Y., Zhou, R., Kim, H., Shi, X., Abeysirigunawardena, S. C., … Ha, T. (2014). An improved surface passivation method for single-molecule studies. Nature Methods, 11(12), 1233–1236. https://doi.org/10.1038/nmeth.3143

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