An improved test for Africanized honeybee mitochondrial DNA

  • Crozier Y
  • Koulianos S
  • Crozier R
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Abstract

Mitochondrial DNA derived from Apis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks a BglII restriction site found in other types of honeybee. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplified product with BglII yields contrasting patterns between Africanized and other honeybees.

Author-supplied keywords

  • Africanized honeybee
  • Apis mellifera scutellata
  • BglII
  • mitochondrial DNA
  • polymerase chain reaction
  • restriction fragment length polymorphism

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Authors

  • Y. C. Crozier

  • S. Koulianos

  • R. H. Crozier

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