Influenza A virus progeny vRNP trafficking in live infected cells studied with the virus-encoded fluorescently tagged PB2 protein

  • Avilov S
  • Moisy D
  • Naffakh N
 et al. 
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Dynamic studies of influenza virus infection in the live cells are limited because of the lack of appropriate methods for non-invasive detection of the viral components. Using the split-GFP strategy, we have recently developed and characterized an unimpaired recombinant influenza A virus encoding a tagged PB2 subunit of RNA-dependent RNA polymerase, which enabled continuous real-time visualization of the viral ribonucleoproteins (vRNPs) in living cells (Avilov, Moisy, Munier, Schraidt, Naffakh and Cusack [12]). Here, using this virus, we studied vRNP trafficking and interaction with Rab11 in the context of quasi-wild type infection. In agreement with recent reports, we observed that upon nuclear export, progeny vRNPs accumulate in the particles containing Rab11, a multifunctional protein involved in vesicle trafficking which resides at recycling endosomes. Fluorescence resonance energy transfer microscopy indicated a distance

Author-supplied keywords

  • Influenza virus
  • Live cell imaging
  • RNA-dependent RNA polymerase
  • Rab11
  • Ribonucleoprotein
  • Traffic

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