Inhibitory effect of some methanol plant extracts on dihydrofolate reductase

  • Gholamhoseinian A
  • Mohammadi A
  • Noroozi S
 et al. 
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Abstract

Cancer claims the lives of more than 6 million people each year globally. Current treatment approaches have yielded significant progress in the fight against cancer, but the incidence of certain types of cancer continues to rise. Dihydrofolate reductase (DHFR) is a key target for cancer chemotherapeutic agents. In this study, some plant exts. were evaluated for their inhibitory activity against DHFR. One hundred species of plants were collected or purchased from the medicinal herbal markets and identified botanically. Methanol exts. were prepd. by maceration. DHFR activity was assayed spectrophotometrically by monitoring the decrease in absorbance at 340 nm. Km and Vmax of the enzyme were detd. using Lineweaver-Burk graphs. Among 100 exts. prepd., methanol exts. of Terminalia chebula and Quercus infectoria showed 100% inhibitory activity while another 11 exts. demonstrated more than 10% inhibitory effect on DHFR. Lineweaver-Burk plots revealed a non-competitive mode of inhibition for both active plants. Under std. condition, Km value for the enzyme was 1.2 μmol and Vmax was 0.01 μmol min-1. Ki value for T. chebula was 1.5 μmol and for Q. infectoria was 3.33 μmol. Data indicated that both T. chebula and Q. infectoria plants may be good candidates for therapeutic use. [on SciFinder(R)]

Author-supplied keywords

  • Dihydrofolate reductase
  • Inhibition
  • Quercus infectoria
  • Terminalia chebula

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