Inhibitory effects of halothane on the thermogenic pathway in brown adipocytes: Localization to adenylyl cyclase and mitochondrial fatty acid oxidation

  • Ohlson K
  • Shabalina I
  • Lennström K
 et al. 
  • 14


    Mendeley users who have this article in their library.
  • 16


    Citations of this article.


Volatile anesthetics such as halothane efficiently inhibit nonshivering thermogenesis as well as the cellular manifestation of that phenomenon: norepinephrine-induced respiration in brown adipocytes. To identify the molecular site(s) of action of such anesthetics, we have examined the effect of halothane on the sequential intracellular steps from the interaction of norepinephrine with isolated brown adipocytes to the stimulation of mitochondrial respiration (=thermogenesis). We did not identify an inhibition at the level of the adrenergic receptors, but a first site of inhibition was identified as the generation of cAMP by adenylyl cyclase; this led to inhibition of norepinephrine-induced expression of the uncoupling protein-1 (UCP1) gene and reduced norepinephrine-induced lipolysis as secondary effects. Although an inhibition of lipolysis in itself would inhibit thermogenesis, circumvention of this inhibition revealed that a second, postlipolytic, site of inhibition existed: halothane also inhibited the stimulatory effect of exogenous fatty acids on cellular respiration. This inhibition was independent of the presence of UCP1 in the mitochondria of the cells and was thus not directly on the thermogenic uncoupling mechanism. Since not only fatty acid oxidation but also pyruvate oxidation were inhibited by halothane in isolated mitochondria, whereas glycerol-3-phosphate oxidation was not, the second site of action of halothane, evident when cyclase/lipolytic inhibition was circumvented, was located to the respiratory chain, complex I. The results thus explain the inhibition of nonshivering thermogenesis by identifying two sites of action of halothane in brown adipocytes. In addition, the results may open for new formulations of the molecular background to anesthesia. © 2004 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Adenylyl cyclase
  • Brown adipose tissue
  • FCCP
  • Fatty acid oxidation
  • Halothane
  • Thermogenesis
  • UCP1
  • Volatile anesthetics
  • carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone
  • uncoupling protein 1

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Kerstin B.E. Ohlson

  • Irina G. Shabalina

  • Katarina Lennström

  • Emma C. Backlund

  • Nina Mohell

  • Gennady E. Bronnikov

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free