Injury in the spinal cord may produce cell death in the brain

  • Lee B
  • Lee K
  • Kim U
 et al. 
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Abstract

Functional deficits after spinal cord injury have originated not only from the direct physical damage itself, but from the secondary biochemical and pathological changes. Apoptotic cell death has been seen around the periphery of an injured site and has been known to ultimately progress to necrosis and infarction. We have initiated the present study focusing on the role of apoptosis in the secondary injury of the brain after acute spinal cord injury (SCI), and conducted a series of experiments, the study examining the morphological changes in the brain following the spinal injury. Under pentobarbital anesthesia, male Sprague-Dawley rats were subjected to SCI model. Rats were laminectomized and SCI was induced using NYU spinal impactor at T9 segment. The behavioral test was performed. Electrophysiologically, motor evoked potentials (MEPs) were recorded. The animals were subjected to morphological study at 12, 24, 48, 72 h, and 1 week, postoperatively. Locomotor deficits were observed after SCI, and changes in the amplitudes and latencies of the MEPs were observed. The morphological changes were evidenced by terminal TUNEL staining and Calbindin-D28Kimmunohistochemistry. The TUNEL-positive cells were located at the brain motor cortex after SCI. TUNEL-positive cells were seldom found 4 h after injury. In addition, Calbindin-D28Kimmunoreactive neurons were observed in the motor cortex after injury. These results suggest that apoptosis may play an important role in the pathophysiology of the brain motor cortex following acute spinal cord injury and functions that were deteriorated after SCI may be related to these electrophysiological and morphological changes. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Behavior
  • Brain
  • Calbindin
  • Cell death
  • Motor system and sensorimotor integration
  • Spinal cord and brainstem
  • Spinal cord injury
  • TUNEL

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Authors

  • Bae Hwan Lee

  • Kyung Hee Lee

  • Un Jeng Kim

  • Do Heum Yoon

  • Jin Hun Sohn

  • Sang Sup Choi

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