Journal article

The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils.

Winternitz C, Jackson J, Burt H ...see all

Rheumatology international, vol. 16 (1996) pp. 101-107

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Monoclinic calcium pyrophosphate dihydrate (m-CPPD) crystals were synthesized and characterized using physical methods, IgG binding to m-CPPD crystals was quantitated, and the effect of IgG or plasma opsonization on m-CPPD-induced neutrophil activation was determined. Adsorption of IgG to crystals was measured using fluorescent-labelled FITC-IgG. Neutrophil activation by uncoated m-CPPD and crystals precoated with IgG or plasma was measured using luminol-enhanced chemiluminescence, superoxide anion generation, and myeloperoxidase release. m-CPPD bound small (compared to triclinic CPPD) but significant amounts of IgG and induced a strong activation of neutrophils at low concentrations of crystals. The rate and extent of chemiluminescence, superoxide anion production, and degranulation was not affected by precoating m-CPPD crystals with IgG during the early phase of neutrophil responses, but was inhibited by the precoating of crystals with plasma.

Author-supplied keywords

  • Adsorption
  • Calcium Pyrophosphate
  • Calcium Pyrophosphate: blood
  • Calcium Pyrophosphate: chemistry
  • Calcium Pyrophosphate: pharmacology
  • Cell Degranulation
  • Cell Degranulation: drug effects
  • Crystallization
  • Humans
  • Immunoglobulin G
  • Immunoglobulin G: metabolism
  • Luminescent Measurements
  • Neutrophils
  • Neutrophils: cytology
  • Neutrophils: metabolism
  • Neutrophils: physiology
  • Particle Size
  • Protein Binding
  • Solubility
  • Superoxides
  • Superoxides: metabolism
  • X-Ray Diffraction

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  • PUI: 26317048
  • ISSN: 0172-8172
  • SGR: 0029836502
  • SCOPUS: 2-s2.0-0029836502
  • PMID: 8893374


  • Charles Ilderton Winternitz

  • John K. Jackson

  • Helen M. Burt

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