R. Chandrashekar et.al / IJIPSR / 2 (5), 2014, 986-992 ISSN (online) 2347-2154 Available online: www.ijipsr.com May Issue 986 FTIR spectroscopy analysis of gossypol from Bt and non-Bt cotton seed extracts www.ijipsr.com Abstract The present paper dealt gossypol content in Bt and non-Bt cotton seed extracts using FTIR spectroscopy with a NaCl transmission cell. The wavelengths used were selected by spiking clean cottonseed extracts to gossypol concentrations of 0–5% and noting the regions of maximal absorbance. Transmittance values from the wavelength regions 3600– 2520 and 1900–800 cm −1 and a partial least squares (PLS) method were used to derive FTIR spectroscopic calibration models for cottonseed extracts. The coefficients of determination (R2) for the models were computed by comparing the results from the FTIR spectroscopy against those obtained by AOCS method Ba 8-78. The R2were 0.9511, 0.9116, and 0.9363 for Bt and non-Bt cottonseed extracts. The SE of calibration was 0.042, 0.009, and 0.060, respectively. The calibration models were cross-validated within the same set of extracts samples. The SD of the difference for repeatability and accuracy of the FTIR method were better than those for the chemical method. With its speed (ca.2min) and ease of data manipulation, FTIR spectroscopy is a useful alternative to standard wet chemical methods for rapid and routine determination of gossypol in process and/or quality control for cottonseed extracts.
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