The patterning of cardiac myocytes on a micron scale (-5 pm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca2+ transition over the pattern, and thus simultaneously contracted. The dynamic change of the Ca2+ concentration within the patterned tissue was analyzed quantitatively during successive contraction and relaxation using a Nipkow-type high-speed confocal microscope. © 2003 Wiley Periodicals, Inc.
CITATION STYLE
Kaji, H., Takoh, K., Nishizawa, M., & Matsue, T. (2003). Intracellular Ca2+ imaging for micropatterned cardiac myocytes. Biotechnology and Bioengineering, 81(6), 748–751. https://doi.org/10.1002/bit.10521
Mendeley helps you to discover research relevant for your work.