Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses.

  • Raz E
  • Carson D
  • Parker S
 et al. 
  • 35

    Readers

    Mendeley users who have this article in their library.
  • 489

    Citations

    Citations of this article.

Abstract

The skin and mucous membranes are the anatomical sites were most viruses are first encountered by the immune system. Previous experiments have suggested that striated muscle cells are unique among mammalian cell types in their capacity to take up and express free DNA in the absence of a viral vector or physical carrier. However, we have found that mice injected into the superficial skin with free (naked) plasmid DNA encoding the influenza nucleoprotein gene had discrete foci of epidermal and dermal cells, including cells with dendritic morphology, that contained immunoreactive nucleoprotein antigen. A single intradermal administration of 0.3-15 micrograms of free plasmid DNA induced anti-nucleoprotein-specific antibody and cytotoxic T lymphocytes that persisted for at least 68-70 weeks after vaccination. Intradermal gene administration induced higher antibody titers than did direct gene injection into skeletal muscle and did not cause local inflammation or necrosis. Compared with control animals, the gene-injected mice were resistant to challenge with a heterologous strain of influenza virus. These results indicate that the cells of the skin can take up and express free foreign DNA and induce cellular and humoral immune responses against the encoded protein. We suggest that DNA uptake by the skin-associated lymphoid tissues may play a role in the induction of cytotoxic T cells against viruses and other intracellular pathogens.

Author-supplied keywords

  • Amino Acid Sequence
  • Animals
  • Avian Sarcoma Viruses
  • Base Sequence
  • Cytomegalovirus
  • Cytotoxicity, Immunologic
  • DNA, Viral
  • DNA, Viral: administration & dosage
  • DNA, Viral: immunology
  • Genetic Vectors
  • Immunity, Cellular
  • Immunization
  • Immunization: methods
  • Influenza A virus
  • Influenza A virus: genetics
  • Influenza A virus: immunology
  • Lung
  • Lung: microbiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nucleoproteins
  • Nucleoproteins: genetics
  • Nucleoproteins: immunology
  • Oligodeoxyribonucleotides
  • Orthomyxoviridae Infections
  • Orthomyxoviridae Infections: immunology
  • Orthomyxoviridae Infections: prevention & control
  • Plasmids
  • Plasmids: administration & dosage
  • Polymerase Chain Reaction
  • Skin
  • Skin: immunology
  • Spleen
  • Spleen: immunology
  • T-Lymphocytes
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes, Cytotoxic: immunology
  • T-Lymphocytes: immunology

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • E Raz

  • D A Carson

  • S E Parker

  • T B Parr

  • A M Abai

  • G Aichinger

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free