Inverted DNA Repeats: a Source of Eukaryotic Genomic Instability

  • Gordenin D
  • Lobachev K
  • Degtyareva N
 et al. 
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Abstract

While inverted DNA repeats are generally acknowledged to be an important source of genetic instability in prokaryotes, relatively little is known about their effects in eukaryotes. Using bacterial transposon Tn5 and its derivatives, we demonstrate that long inverted repeats also cause genetic instability leading to deletion in the yeast Saccharomyces cerevisiae. Furthermore, they induce homologous recombination. Replication plays a major role in the deletion formation. Deletions are stimulated by a mutation in the DNA polymerase 8 gene (pol3). The majority of deletions result from imprecise excision between small (4-to 6-bp) repeats in a polar fashion, and they often generate quasipalindrome structures that subsequently may be highly unstable. Breakpoints are clustered near the ends of the long inverted repeats (

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Authors

  • Dmitry A Gordenin

  • Kirill S Lobachev

  • Natasha P Degtyareva

  • Anna L Malkova

  • Edward Perkins

  • Michael A Resnick2

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