Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

  • Tang W
  • Li Z
  • Zhao H
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We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).

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  • Weng Lin Tang

  • Zhi Li

  • Huimin Zhao

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