Involvement of estrogen receptor beta in terminal differentiation of mammary gland epithelium.

  • Förster C
  • Mäkela S
  • Wärri A
 et al. 
  • 37

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Abstract

The mammary glands of prepubertal estrogen receptor (ER)beta-- mice are morphologically indistinguishable from those of WT littermates. It appears that, although ERbeta is expressed in the mouse mammary gland, it is not involved in ductal growth of the gland. In this study, we examined the possibility that ERbeta has a role in the differentiated function of the mammary gland. Pregnancy is rare in ERbeta-- mice, but an intensive breeding program produced seven pregnant ERbeta-- mice, of which five did not eat their offspring and continued to successful lactation. Histomorphological comparison of lactating glands revealed that alveoli were larger and there was less secretory epithelium in ERbeta-- than in WT mice. Ultrastructural analysis showed abundant milk droplets and normal apical villi in the luminal epithelial cells, but the extracellular matrix and lamina basalis were reduced, and very frequently the interepithelial cell space was increased. Levels of the adhesion molecules, E-cadherin, connexin 32, occludin, and integrin alpha2 were reduced, and no zona occludens was detectable. In addition, there was widespread expression of the proliferation marker, Ki-67, in luminal epithelial cells in ERbeta-- but not in WT mice. These findings suggest a role for ERbeta in organization and adhesion of epithelial cells and hence for differentiated tissue morphology. We speculate that, because a reduced risk for breast cancer is conferred on women who breast-feed at an early age, ERbeta could contribute to this risk reduction by facilitating terminal differentiation of the mammary gland.

Author-supplied keywords

  • Animals
  • Cadherins
  • Cadherins: analysis
  • Cell Differentiation
  • Cell Polarity
  • Epithelial Cells
  • Epithelial Cells: cytology
  • Epithelial Cells: secretion
  • Estrogen Receptor beta
  • Female
  • Fertility
  • Gap Junctions
  • Gap Junctions: ultrastructure
  • Integrin alpha2
  • Integrin alpha2: analysis
  • Integrin alpha6
  • Integrin alpha6: analysis
  • Lactation
  • Mammary Glands, Animal
  • Mammary Glands, Animal: cytology
  • Mammary Glands, Animal: growth & development
  • Mice
  • Mice, Knockout
  • Pregnancy
  • Receptors, Estrogen
  • Receptors, Estrogen: deficiency
  • Receptors, Estrogen: genetics
  • Receptors, Estrogen: physiology
  • Tight Junctions
  • Tight Junctions: ultrastructure

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Authors

  • Carola Förster

  • Sari Mäkela

  • Anni Wärri

  • Silke Kietz

  • David Becker

  • Kjell Hultenby

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