Isolation of the Antibiotic Reductiomycin from a Terrestrial Bacterium Streptomyces sp.

Abstract

ABSTRAK Penelitian ini bertujuan melakukan isolasi dan penentuan aktivitas mikrobial senyawa bioaktif reduktiomisin dari bakteri Streptomyces sp. Ank181 daratan (terrestrial). Penelitian ini dilaksanakan di laboratorium kimia organik, Institute of Organic and Biomoleculare Chemistry, University of Goettingen, Germany. Isolat dan subkultur agar bakteri Streptomyces sp. Ank181 diperoleh dari koleksi sampel genus Streptomyces Professor Dr. H. Anke, Institute for Biotechnology and Drug Research, Kaiserslautern, Germany. Struktur reduktiomisin dalam penelitian ini dielusidasi berdasarkan data spektroskopi dan spektrometri massa. Tiga senyawa metabolit sekunder telah berhasil diisolasi dan diidentifikasi dari bakteri tanah genus Streptomyces sp. Ank181, yaitu reduktiomisin, asam 2,3-dihidroksibenzoat, dan asam indole-3-karboksilat. Hasil uji antimikroba menunjukkan bahwa reduktiomisin bersifat bioaktif terhadap bakteri, jamur dan sitotoksik terhadap Artemia salina. ____________________________________________________________________________________________________________________ Kata kunci: antibiotik, reduktiomisin, Streptomyces sp., antimikroba ABSTRACT This research was aimed to isolate and screen antimicrobial activity of reductiomycin from terrestrial bacterium Streptomyces sp. Ank181. Isolate and agar subculture of bacterium Streptomyces sp. Ank181 were received from a collection of Professor Dr. H. Anke, Institute for Biotechnology and Drug Research, Keiserslautern, Germany. Reductiomycin structure was elucidated based on spectroscopic and mass spectrometry data. In this research, three secondary metabolites has been isolated and identified from terrestrial bacterium Streptomyces sp. Ank181, namely reductiomycin, 2,3-dihydroxybenzoic acid and indole-3-carboxylic acid. Antimicrobial bioassay of reductiomycin showed activities against bacteria, fungi and cytotoxic against Artemia salina.