Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae).

  • Jain S
  • McPhee K
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PREMISE OF THE STUDY: Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. •

METHODS AND RESULTS: Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. •

CONCLUSIONS: The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species.

Author-supplied keywords

  • and rich source of
  • comparative mapping
  • expressed sequence tags
  • in temperate regions of
  • is an important grain
  • legume grown
  • lentil
  • marker-assisted selection
  • pea
  • pisum sativum l
  • protein and contribute to
  • seeds are a cheap
  • synteny
  • the nutritional
  • the world because its

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  • Shalu Jain

  • Kevin E McPhee

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