BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, vol. 49, issue 4 (2006) pp. 537-545
Protoplasts were isolated from cotton microspores by hydrolysing the
microspore wall with snailase and driselase; the snailase was superior
to the driselase and the optimum concentration was 0.5%. Sucrose
was used as an osmotic regulator, the optimum concentration being
20%. The protoplasts obtained by this method were cultured in K3
medium containing 0.5 mg 2,4-D, 1.0 mg 2iP and 0.5 mg kinetin/litre.
The first cell divisions were observed after 3-4 days and the second
after 10 days. No further cell division was observed, indicating
that the culture conditions require further improvement.
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