Isolation and Culture of Soybean (Glycine max L. Merrill) Microspores and Pollen Grains

  • Lia Rosane Rodrigues1 B
  • Bodanese-Zanettini1 M
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Abstract

Protoplasts were isolated from cotton microspores by hydrolysing the microspore wall with snailase and driselase; the snailase was superior to the driselase and the optimum concentration was 0.5%. Sucrose was used as an osmotic regulator, the optimum concentration being 20%. The protoplasts obtained by this method were cultured in K3 medium containing 0.5 mg 2,4-D, 1.0 mg 2iP and 0.5 mg kinetin/litre. The first cell divisions were observed after 3-4 days and the second after 10 days. No further cell division was observed, indicating that the culture conditions require further improvement.

Author-supplied keywords

  • Androgenesis
  • Glycine max
  • microspore culture
  • microspore embryogenesis
  • microspore isolation
  • soybean

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Authors

  • Bianca de Camargo Forte1 Lia Rosane Rodrigues1

  • Maria Helena Bodanese-Zanettini1

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