Isolation of human NK cells and generation of LAK activity.

  • Whiteside T
  • 43

    Readers

    Mendeley users who have this article in their library.
  • 3

    Citations

    Citations of this article.

Abstract

This unit describes the preparation of natural killer (NK) cells from normal human peripheral blood and their incubation in vitro in the presence of interleukin 2 (IL-2) to yield lymphokine-activated killer (LAK) cells. A protocol is presented for isolating highly purified NK cell populations from PBMC, and another method presents steps for generating LAK cells from these purified NK cells via IL-2 stimulation. An alternate protocol describes the generation of LAK cells directly from whole, unseparated PBMC preparations instead of purified NK populations. The caveat with the alternate protocol is that LAK activity generated in this manner represents the total cytotoxic potential of all LAK precursor cells--i.e., all those PBMC that are capable of responding to IL-2 by up-regulation of cytotoxicity against NK-resistant targets. In PBMC, LAK precursor cells are found among subpopulations of both NK cells and T lymphocytes.

Author-supplied keywords

  • Cell Separation
  • Cell Separation: methods
  • Humans
  • Interleukin-2
  • Interleukin-2: immunology
  • Interleukin-2: pharmacology
  • Killer Cells, Lymphokine-Activated
  • Killer Cells, Lymphokine-Activated: cytology
  • Killer Cells, Lymphokine-Activated: drug effects
  • Killer Cells, Lymphokine-Activated: immunology
  • Killer Cells, Natural
  • Killer Cells, Natural: cytology
  • Killer Cells, Natural: drug effects
  • Killer Cells, Natural: immunology
  • Leukocytes, Mononuclear
  • Leukocytes, Mononuclear: cytology
  • Leukocytes, Mononuclear: drug effects
  • Leukocytes, Mononuclear: immunology

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • T L Whiteside

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free