Transport of preproteins into the mitochondrial matrix is mediated by the presequence translocase-associated motor (PAM). Three essential subunits of the motor are known: mitochondrial Hsp70 (mtHsp70); the peripheral membrane protein Tim44; and the nucleotide exchange factor Mge1. We have identified the fourth essential subunit of the PAM, an essential inner membrane protein of 18 kD with a J-domain that stimulates the ATPase activity of mtHsp70. The novel J-protein (encoded by PAM18/ YLR008c/TIM14) is required for the interaction of mtHsp70 with Tim44 and protein translocation into the matrix. We conclude that the reaction cycle of the PAM of mitochondria involves an essential J-protein.
CITATION STYLE
Truscott, K. N., Voos, W., Frazier, A. E., Lind, M., Li, Y., Geissler, A., … Pfanner, N. (2003). A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondria. Journal of Cell Biology, 163(4), 707–713. https://doi.org/10.1083/jcb.200308004
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