Kinetics of growth and glucose transport in glucose???limited chemostat cultures of Saccharomyces cerevisiae CBS 8066

  • Postma E
  • Alexander Scheffers W
  • Van Dijken J
  • 35

    Readers

    Mendeley users who have this article in their library.
  • 84

    Citations

    Citations of this article.

Abstract

The glucose transport capacity of Saccharomyces cerevisiae CBS 8066 was studied in aerobic glucose-limited chemostat cultures. Two different transport systems were encountered with affinity constants of 1 and 20 mM, respectively. The capacity of these carriers (Vmax) was dependent on the dilution rate and the residual glucose concentration in the culture. From the residual glucose concentration in the fermenter and the kinetic constants of glucose transport, their in situ contribution to glucose consumption was determined. The sum of these calculated in situ transport rates correlated well with the observed rate of glucose consumption of the culture. The growth kinetics of S. cerevisiae CBS 8066 in glucose-limited cultures were rather peculiar. At low dilution rates, at which glucose was completely respired, the glucose concentration in the fermenter was constant at 110 microM, independent of the glucose concentration in the reservoir. At higher dilution rates, characterized by the occurrence of both respiration and alcoholic fermentation, the residual substrate concentration followed Monod kinetics. In this case, however, the overall affinity constant was dependent on the reservoir glucose concentration.

Author-supplied keywords

  • Crabtree effect
  • chemostat
  • growth kinetics
  • sugar transport
  • yeast

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Erik Postma

  • W. Alexander Scheffers

  • Johannes P. Van Dijken

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free