Label free aptasensor for Lysozyme detection: A comparison of the analytical performance of two aptamers

  • Ocaña C
  • Hayat A
  • Mishra R
 et al. 
  • 32

    Readers

    Mendeley users who have this article in their library.
  • 19

    Citations

    Citations of this article.

Abstract

This work presents a comparison of two different aptamers (Apts) (COX and TRAN) for the detection of a ubiquitous protein Lysozyme (Lys) using Apt-based biosensors. The detection is based on the specific recognition by the Apt immobilized on screen printed carbon electrodes (SPCEs) via diazonium coupling reaction. The quantitative detection of Lys protein was achieved by electrochemical impedance spectroscopy (EIS). A very good linearity and detection limits for the quantitation of Lys were obtained from 0.1 to 0.8. μM and 100. nM using Apt COX and from 0.025 to 0.8. μM and 25. nM using Apt TRAN respectively. The obtained results showed that the developed aptasensors exhibit good specificity, stability and reproducibility for Lys detection. For real application, the aptasensors were tested in wine samples and good recovery rates were recorded in the range from 94.2 to 102% for Lys detection. The obtained recovery rates confirm the reliability and suitability of the developed method in wine matrix. The developed method could be a useful and promising platform for detection of Lys in different applications.

Author-supplied keywords

  • Diazonium chemistry
  • Disposable aptasensor
  • Label free detection
  • Lysozyme
  • Wine samples

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free