In this work, by incorporating a specific DNAzyme sequence into a hairpin aptamer probe, we describe a label-free and sensitive method for electrochemical detection of cytokines using recombinant human IFN-γ as the model analyte. The hairpin aptamer probes are immobilized on a gold electrode through self-assembly. The presence of IFN-γ opens the hairpin structure and forms the hemin/G-quadruplex peroxidase-mimicking DNAzyme with subsequent addition of hemin. The peroxidase-mimicking DNAzyme catalyzes the electro-reduction of H2O2 and amplifies the current response for IFN-γ detection, which enables the monitoring of IFN-γ at the sub-nanomolar level. The proposed sensor also shows high selectivity towards the target analyte. Our strategy thus opens new opportunities for label-free and amplified detection of different types of cytokines. © The Royal Society of Chemistry 2012.
CITATION STYLE
Zhang, H., Jiang, B., Xiang, Y., Chai, Y., & Yuan, R. (2012). Label-free and amplified electrochemical detection of cytokine based on hairpin aptamer and catalytic DNAzyme. Analyst, 137(4), 1020–1023. https://doi.org/10.1039/c2an15962g
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