A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods

  • Mayer K
  • Lee S
  • Liao H
 et al. 
  • 13

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Abstract

Robust gold nanorod substrates were fabricated for refractive index sensing based on localized surface plasmon resonance (LSPR). The substrate sensitivity was 170 nm/RIU with a figure of merit of 1.3. To monitor biomolecular interactions, the nanorod surfaces were covered with a self-assembled monolayer and conjugated to antibodies by carbodiimide cross-linking. Interactions with a specific secondary antibody were monitored through shifts in the LSPR spectral extinction peak. The resulting binding rates and equilibrium constant were in good agreement with literature values for an antibody-antigen system. The nanorod LSPR sensors were also shown to be sensitive and specific. These results demonstrate that given a sufficiently stable nanoparticle substrate with a well defined chemical interface, LSPR sensing yields similar results to the surface plasmon resonance technique, yet with much simpler instrumentation.

Author-supplied keywords

  • Biosensing Techniques/*methods
  • Crystallization/methods
  • Gold/*chemistry
  • Immunoassay/*methods
  • Macromolecular Substances/chemistry
  • Materials Testing
  • Molecular Conformation
  • Nanotechnology/methods
  • Nanotubes/*chemistry/*ultrastructure
  • Particle Size
  • Staining and Labeling
  • Surface Plasmon Resonance/*methods
  • Surface Properties
  • Titanium/*chemistry

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Authors

  • K M Mayer

  • S Lee

  • H Liao

  • B C Rostro

  • A Fuentes

  • P T Scully

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