Localized substance delivery to single cell and 4D imaging of its uptake using a flow channel with a lateral aperture

  • Terao K
  • Okonogi A
  • Fuke A
 et al. 
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Abstract

We have developed a novel microfluidic device for space- and
time-resolved (4D) visualization of intracellular events when a cell
surface is partially exposed to external stimuli. The device, fabricated
using 3D rotational inclined UV lithography of photoresist SU-8,
consists of a cell-containing chamber and a flow channel separated by a
thin vertical wall having a lateral micrometer aperture smaller than a
cell. A cell is first immobilized on the aperture by suction from the
flow channel using a syringe pump, and a chemical stimulant is then fed
to the channel so that only the cell surface bounded by the aperture is
subjected to the stimulus without leakage to other part of the cell
surface. The subsequent lateral signal propagation inside the cell can
be visualized using high-speed fluorescence confocal microscopy. As an
experimental demonstration of the device, 2-NBDG (fluorescence glucose
analog) intake into a mouse insulinoma cell, MIN6m9, was visualized in
4D resolution.

Author-supplied keywords

  • 4D imaging
  • Localized substance delivery
  • Microfluidic channel
  • Multidirectional UV lithography
  • Single cell analysis

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Authors

  • Kyohei Terao

  • Atsuhito Okonogi

  • Ariko Fuke

  • Teru Okitsu

  • Takaaki Suzuki

  • Masao Washizu

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