Long-term storage of sperm in liquid nitrogen is a valuable technique for genetic resources preservation (Kopeika et al. 2007). The research on fish sperm cryopreservation has achieved great advances since the first successful sperm cryopreservation in herring (Blaxter 1953). It provides many benefits such as ease of global germplasm shipping and supply (Tiersch et al. 2004), selective breeding and hybridization with desirable characteristics (Henderson- Arzapalo et al. 1994), and conservation of genetic diversity (Van der Walt et al. 1993; Tiersch et al. 2000; Ohta et al. 2001). Furthermore, a frozen sperm bank could maintain the continuous and stable supply of gametes for hatchery seed production or laboratory experimentation. Because of the advantages of this technique, fish sperm of over 200 freshwater and 40 marine species have been cryopreserved successfully (Gwo 2000).
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