In ants, aggregation is mainly based on the at- traction between individuals. We confirmed the existence of ground marking in Lasius niger and demonstrated its in- fluence on the aggregative behaviour of ants: the place of the gathering is leading by the existence of a ground marking which favours clustering by increasing the rate of the process but does not change the aggregation level. This factor could play a role in the choice of a new nest site and in the spatial distribution of ants inside the nest. This marking, presuming to have a long duration, could represent an external memory which could play an important role in various situations such as the choice of new nest or the reorganisation of ants after a disturbance.
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