The use of MassARRAY technology for high throughput genotyping.

  • Jurinke C
  • van den Boom D
  • Cantor C
 et al. 
  • 43

    Readers

    Mendeley users who have this article in their library.
  • 137

    Citations

    Citations of this article.

Abstract

This chapter will explore the role of mass spectrometry (MS) as a detection method for genotyping applications and will illustrate how MS evolved from an expert-user-technology to a routine laboratory method in biological sciences. The main focus will be time-of-flight (TOF) based devices and their use for analyzing single-nucleotide-polymorphisms (SNPs, pronounced snips). The first section will describe the evolution of the use of MS in the field of bioanalytical sciences and the protocols used during the early days of bioanalytical MALDI TOF mass spectrometry. The second section will provide an overview on intraspecies sequence diversity and the nature and importance of SNPs for the genomic sciences. This is followed by an exploration of the special and advantageous features of mass spectrometry as the key technology in modern bioanalytical sciences in the third chapter. Finally, the fourth section will describe the MassARRAY technology as an advanced system for automated high-throughput analysis of SNPs.

Author-supplied keywords

  • dna chip
  • genomics
  • high-throughput
  • maldi mass spectrometry
  • snp

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Christian Jurinke

  • Dirk van den Boom

  • Charles R Cantor

  • Hubert Köster

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free