Mast cell activation on patterned lipid bilayers of subcellular dimensions

  • Orth R
  • Wu M
  • Holowka D
 et al. 
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This work presents an original and straightforward technique for antibody
immobilization onto a surface, keeping the antibody in a biologically
reactive configuration. Self-assembly of molecular monolayers and
plasma-based colloidal lithography were combined to create chemical
nanopatterns on the surface of a biosensing device. This technique
was employed to create an array of 100 nm wide motifs having a hexagonal
2-D crystalline structure, characterized by COOH-terminated nanospots
in a CH3-terminated matrix. The quality control of the chemical nanopattern
was carried out by combining atomic force microscopy, ellipsometry,
and contact angle measurements. Enzyme-linked immunosorbent assay
experiments were set up showing that the COOH/CH3 nanopatterned surface
constrains the immobilization of the antibodies in a biologically
reactive configuration, thus significantly improving the device performances
as compared to those of more conventional nonpatterned COOH-terminated
or CH3-terminated surfaces.

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