Mature dendritic cells use endocytic receptors to capture and present antigens

  • Platt C
  • Ma J
  • Chalouni C
 et al. 
  • 262

    Readers

    Mendeley users who have this article in their library.
  • 115

    Citations

    Citations of this article.

Abstract

In response to inflammatory stimuli, dendritic cells (DCs) trigger the process of maturation, a terminal differentiation program required to initiate T-lymphocyte responses. A hallmark of maturation is down-regulation of endocytosis, which is widely assumed to restrict the ability of mature DCs to capture and present antigens encountered after the initial stimulus. We found that mature DCs continue to accumulate antigens, especially by receptor-mediated endocytosis and phagocytosis. Internalized antigens are transported normally to late endosomes and lysosomes, loaded onto MHC class II molecules (MHCII), and then presented efficiently to T cells. This occurs despite the fact that maturation results in the general depletion of MHCII from late endocytic compartments, with MHCII enrichment being typically thought to be a required feature of antigen processing and peptide loading compartments. Internalized antigens can also be cross-presented on MHC class I molecules, without any reduction in efficiency relative to immature DCs. Thus, although mature DCs markedly down-regulate their capacity for macropinocytosis, they continue to capture, process, and present antigens internalized via endocytic receptors, suggesting that they may continuously initiate responses to newly encountered antigens during the course of an infection.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • C. D. Platt

  • J. K. Ma

  • C. Chalouni

  • M. Ebersold

  • H. Bou-Reslan

  • R. A. D. Carano

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free