Measuring the flexibility of immunoglobulin by gold nanoparticles

  • Steven Huang G
  • Chen Y
  • Yeh H
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Abstract

We measured the flexibility of Fab and Fc arms of immunoglobulin using gold nanoparticles (GNPs). Enzyme-linked immunosorbent assay was performed to measure the affinity of anti-5 nm GNP antiserum against various sizes of GNPs. The flexibility of Fc was also measured by electron microscopy. The restricted binding affinity indicated that only a very limited amount of freedom was allowed for the Fab-Fab hinge, while Fab-Fc showed a much larger degree of freedom.

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Authors

  • G. Steven Huang

  • Yu Shiun Chen

  • Hsiao Wei Yeh

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