Measuring low levels of protein aggregation by sedimentation velocity

  • Gabrielson J
  • Arthur K
  • 17


    Mendeley users who have this article in their library.
  • 35


    Citations of this article.


The required performance of an analytical method depends on the purpose for which it will be used. As a methodology matures, it may find new application, and the performance demands placed on the method can increase. Sedimentation velocity analytical ultracentrifugation (SV-AUC) has a long and distinguished history with important contributions to molecular biology. Now the technique is transitioning into industrial settings, and among them, SV-AUC is now used to quantify the amount of protein aggregation in biopharmaceutical protein products, often at levels less than 1% of the total protein mass. In this paper, we review recent advances to SV methodology which have been shown to improve quantitation of protein aggregation. Then we discuss the performance of the SV method in its current state, with emphasis on the precision and quantitation limit of the method, in the context of existing industrial guidance on analytical method performance targets for quantitative methods. © 2011 Elsevier Inc.

Author-supplied keywords

  • Aggregate quantitation
  • Analytical ultracentrifugation
  • Method precision
  • Protein aggregation
  • Sedimentation velocity
  • Therapeutic proteins

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • John P. Gabrielson

  • Kelly K. Arthur

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free