Skip to content
Journal article

Mechanism of up-regulation of human Toll-like receptor 3 secondary to infection of measles virus-attenuated strains

Tanabe M, Kurita-Taniguchi M, Takeuchi K, Takeda M, Ayata M, Ogura H, Matsumoto M, Seya T ...see all

Biochemical and Biophysical Research Communications, vol. 311, issue 1 (2003) pp. 39-48

  • 20

    Readers

    Mendeley users who have this article in their library.
  • 70

    Citations

    Citations of this article.
  • 3.0k

    Views

    ScienceDirect users who have downloaded this article.
Sign in to save reference

Abstract

PolyI:C, a synthetic double-stranded (ds)RNA, and viruses act on cells to induce IFN-?? which is a key molecule for anti-viral response. Although dsRNA is a virus-specific signature and a ligand for human Toll-like receptor 3 (TLR3), largely uncharacterized multiple pathways associate virus-mediated IFN-?? induction. Here, we demonstrated that laboratory-adapted but not wild-type strains of measles virus (MV) up-regulated TLR3 expression both in dendritic cells and epithelial cell line A549. The kinetics experiments with the laboratory MV strain revealed that TLR3 was induced late compared to IFN-?? and required new protein synthesis. Furthermore, neutralizing antibodies against IFN-?? or IFNAR (Interferon-??/?? receptor) suppressed MV-induced TLR3 induction, indicating that type I IFN, IFN-??/??, is critical for MV-mediated TLR3 induction. Yet, a recently identified virus-inducible IFN, the IFN-??, did not contribute to TLR3 expression. A virus-responsive element that up-regulates TLR3 was identified in the TLR3-promoter region by reporter gene experiments. The ISRE, a recently reported site for IFN-?? induction, but not STAT binding site, located around -30bp of TLR3 promoter responded to MV to induce TLR3 expression. This further indicates the importance of type I IFN for TLR3 up-regulation in the case of viral infection. In HeLa and MRC5 cells, augmented production of IFN-?? was observed in response to dsRNA when TLR3 had been induced beforehand. Thus, the MV-induced expression of TLR3 may reflect amplified IFN production that plays a part in host defense to viral infection. ?? 2003 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • IFN-??/??
  • ISRE
  • Measles virus
  • Toll-like receptor 3
  • Transcriptional regulation

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Masako Tanabe

  • Mitsue Kurita-Taniguchi

  • Kaoru Takeuchi

  • Makoto Takeda

  • Minoru Ayata

  • Hisashi Ogura

Cite this document

Choose a citation style from the tabs below