Membrane topology of the L-rhamnose-H+ transport protein (RhaT) from enterobacteria

  • Tate C
  • Henderson P
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The L-rhamnose-H+ symporter (RhaT) is a 344-amino acid integral membrane protein, found in many Enterobacteria, which couples the uptake of the sugar L-rhamnose with the inward movement of protons. Based on its hydropathy profile and the application of von Heijne's "positive inside" rule (von Heijne, G. (1992) J. Mol. Biol. 225, 487-494), a model of the L-rhamnose-H+ symport protein (RhaT) is proposed containing 10 transmembrane helices with the NH2 and COOH termini in the periplasm. This model was tested by the creation of random beta-lactamase (Bla) fusions. The data from 33 unique, randomly generated, RhaT-Bla fusions and from 5 site-specific fusions supported the proposed topology between transmembrane helices 2-10. However, the localization of the putative first hydrophilic loop and the NH2 terminus was not possible because the beta-lactamase fusions in this region were shown to be unreliable indicators of the topology of RhaT.

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  • SGR: 0027752961
  • PUI: 23953145
  • SCOPUS: 2-s2.0-0027752961
  • ISSN: 00219258
  • PMID: 8262918

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