A membranous form of ICAM-1 on exosomes efficiently blocks leukocyte adhesion to activated endothelial cells

  • Lee H
  • Choi E
  • Kim J
 et al. 
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While intercellular adhesion molecule-1 (ICAM-1) is a transmembrane protein, two types of extracellular ICAM-1 have been detected in cell culture supernatants as well as in the serum: a soluble form of ICAM-1 (sICAM-1) and a membranous form of ICAM-1 (mICAM-1) associated with exosomes. Previous observations have demonstrated that sICAM-1 cannot exert potent immune modulatory activity due to its low affinity for leukocyte function-associated antigen-1 (LFA-1) or membrane attack complex-1. In this report, we initially observed that human cancer cells shed mICAM-1(+)-exosomes but were devoid of vascular cell adhesion molecule-1 and E-selectin. We demonstrate that mICAM-1 on exosomes retained its topology similar to that of cell surface ICAM-1, and could bind to leukocytes. In addition, we show that exosomal mICAM-1 exhibits potent anti-leukocyte adhesion activity to tumor necrosis factor-α-activated endothelial cells compared to that of sICAM-1. Taken together with previous findings, our results indicate that mICAM-1 on exosomes exhibits potent immune modulatory activity. © 2010 Elsevier Inc.

Author-supplied keywords

  • Communicasome
  • Endothelial cell
  • Exosomes
  • ICAM-1
  • Inflammation
  • Microparticles

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  • Hwan Myung Lee

  • Eun Jeong Choi

  • Ji Hyun Kim

  • Yoon Keun Kim

  • Chulhun Kang

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