Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth

  • Sokol N
  • Ambros V
  • 133

    Readers

    Mendeley users who have this article in their library.
  • 293

    Citations

    Citations of this article.

Abstract

Although hundreds of evolutionarily conserved microRNAs have been discovered, the functions of most remain unknown. Here, we describe the embryonic spatiotemporal expression profile, transcriptional regulation, and loss-of-function phenotype of Drosophila miR-1 (DmiR-1). DmiR-1 RNA is highly expressed throughout the mesoderm of early embryos and subsequently in somatic, visceral, and pharyngeal muscles, and the dorsal vessel. The expression of DmiR-1 is controlled by the Twist and Mef2 transcription factors. DmiR-1KO mutants, generated using ends-in gene targeting, die as small, immobilized second instar larvae with severely deformed musculature. This lethality is rescued when a DmiR-1 transgene is expressed specifically in the mesoderm and muscle. Strikingly, feeding triggers DmiR-1KO-associated paralysis and death; starved first instar DmiR-1KO larvae are essentially normal. Thus, DmiR-1 is not required for the formation or physiological function of the larval musculature, but is required for the dramatic post-mitotic growth of larval muscle.

Author-supplied keywords

  • Drosophila
  • Larval development
  • MicroRNA
  • Muscle
  • miR-1

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Nicholas S. Sokol

  • Victor Ambros

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free