This unit describes methods for preparation of glycoproteins metabolically labeled with radioactive sugars, sulfate, and phosphate. Methods for liberation of both N‐ and O‐linked glycans are also described. These protocols can be used to generate materials for characterization of glycoprotein glycans from cultured cells.
CITATION STYLE
Snider, M. D. (2002). Metabolic Labeling of Glycoproteins with Radioactive Sugars. Current Protocols in Cell Biology, 13(1). https://doi.org/10.1002/0471143030.cb0708s13
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