Microarray analysis of gene expression in soybean roots susceptible to the soybean cyst nematode two days post invasion.

  • Khan R
  • Alkharouf N
  • Beard H
 et al. 
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Abstract

Soybean root cells undergo dramatic morphological and biochemical changes during the establishment of a feeding site in a compatible interaction with the soybean cyst nematode (SCN). We constructed a cDNA microarray with approximately 1,300 cDNA inserts targeted to identify differentially expressed genes during the compatible interaction of SCN with soybean roots 2 days after infection. Three independent biological replicates were grown and inoculated with SCN, and 2 days later RNA was extracted for hybridization to microarrays and compared to noninoculated controls. Statistical analysis indicated that approximately 8% of the genes monitored were induced and more than 50% of these were genes of unknown function. Notable genes that were more highly expressed 2 days after inoculation with SCN as compared to noninoculated roots included the repetitive proline-rich glycoprotein, the stress-induced gene SAM22, ss-1,3-endoglucanase, peroxidase, and those involved in carbohydrate metabolism, plant defense, and signaling.

Author-supplied keywords

  • agronomically
  • glycine max
  • heterodera glycines
  • important plants
  • microarray
  • plant-parasitic nematodes attack many
  • plant-pathogen interaction
  • resulting in substantial economic
  • sgmd
  • signaling pathway
  • soybean cyst nema-
  • soybean genomics microarray database
  • syncytium
  • tode

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Authors

  • R Khan

  • N Alkharouf

  • H Beard

  • M Macdonald

  • I Chouikha

  • S Meyer

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