A microfluidic oligonucleotide synthesizer

  • Lee C
  • Snyder T
  • Quake S
  • 124


    Mendeley users who have this article in their library.
  • 51


    Citations of this article.


De novo gene and genome synthesis enables the design of any sequence without the requirement of a pre-existing template as in traditional genetic engineering methods. The ability to mass produce synthetic genes holds great potential for biological research, but widespread availability of de novo DNA constructs is currently hampered by their high cost. In this work, we describe a microfluidic platform for parallel solid phase synthesis of oligonucleotides that can greatly reduce the cost of gene synthesis by reducing reagent consumption (by 100-fold) while maintaining a approximately 100 pmol synthesis scale so there is no need for amplification before assembly. Sixteen oligonucleotides were synthesized in parallel on this platform and then successfully used in a ligation-mediated assembly method to generate DNA constructs approximately 200 bp in length.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Cheng Chung Lee

  • Thomas M. Snyder

  • Stephen R. Quake

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free