Micropropagation of Vitis vinifera L: Towards an improved protocol.

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Nodal explants bearing a single axillary bud, from three cultivars of cultivated grape, viz., 'Thompson seedless', 'Sonaka' and 'Tas-e-Ganesh', were used to initiate shoot cultures on G16 medium containing adenine sulphate, monobasic sodium phosphate, BAP and NAA. Each shoot, developed from an axillary bud, produced a tuft of multiple shoots on a medium containing BAP, calcium pantothenate, monobasic sodium phosphate and IBA. Subculture of the tuft of multiple shoots to an 'elongation medium' resulted in distinct individual shoots. Rooting of shoots and plantlet formation was achieved on IAA-containing liquid medium. A culture procedure for enhanced multiple shoot production and the recovery of complete plants is described. The use of this protocol for the commercial exploitation of cultivated grape is discussed.

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  • Mhatre-Minal A

  • Salunkhe C-K

  • Rao P-S

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