Micropropagation of Vitis vinifera L: Towards an improved protocol.

  • A M
  • C-K S
  • P-S R
  • 2

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

Nodal explants bearing a single axillary bud, from three cultivars of cultivated grape, viz., 'Thompson seedless', 'Sonaka' and 'Tas-e-Ganesh', were used to initiate shoot cultures on G16 medium containing adenine sulphate, monobasic sodium phosphate, BAP and NAA. Each shoot, developed from an axillary bud, produced a tuft of multiple shoots on a medium containing BAP, calcium pantothenate, monobasic sodium phosphate and IBA. Subculture of the tuft of multiple shoots to an 'elongation medium' resulted in distinct individual shoots. Rooting of shoots and plantlet formation was achieved on IAA-containing liquid medium. A culture procedure for enhanced multiple shoot production and the recovery of complete plants is described. The use of this protocol for the commercial exploitation of cultivated grape is discussed.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

There are no full text links

Authors

  • Mhatre-Minal A

  • Salunkhe C-K

  • Rao P-S

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free